{"product_id":"veratox-r5-gliadin-elisa-ricerca-glutine","title":"Veratox R5 Gliadin – ELISA ricerca Glutine","description":"\u003cp data-sourcepos=\"7:1-7:50\"\u003eQuesto kit è ideale per l’analisi quantitativa di:\u003c\/p\u003e\u003cbr\u003e\u003cul data-sourcepos=\"9:1-13:0\"\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"9:1-9:13\"\u003eIngredienti\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"10:1-10:36\"\u003eRisciacqui CIP (Cleaning-in-Place)\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"11:1-11:55\"\u003eProdotti alimentari finiti dichiarati “senza glutine”\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"12:1-13:0\"\u003eScreening di tamponi ambientali\u003c\/li\u003e\n\u003cbr\u003e\n\u003c\/ul\u003e\u003cbr\u003e\u003cbr\u003e\u003cp data-sourcepos=\"5:1-5:89\"\u003e\u003cstrong\u003eTest ELISA R5 per la Ricerca Quantitativa di Glutine (Conforme al Codex Alimentarius)\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp data-sourcepos=\"7:1-7:50\"\u003eQuesto kit è ideale per l'analisi quantitativa di:\u003c\/p\u003e\u003cbr\u003e\u003cul data-sourcepos=\"9:1-13:0\"\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"9:1-9:13\"\u003eIngredienti\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"10:1-10:36\"\u003eRisciacqui CIP (Cleaning-in-Place)\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"11:1-11:55\"\u003eProdotti alimentari finiti dichiarati \"senza glutine\"\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"12:1-13:0\"\u003eScreening di tamponi ambientali\u003c\/li\u003e\n\u003cbr\u003e\n\u003c\/ul\u003e\u003cbr\u003e\u003cp data-sourcepos=\"14:1-14:23\"\u003e\u003cstrong\u003ePrincipio del Test:\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003col data-sourcepos=\"16:1-26:0\"\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"16:1-19:65\"\u003e\n\u003cstrong\u003eEstrazione:\u003c\/strong\u003e La gliadina e altre prolamine da frumento, orzo e segale vengono estratte utilizzando:\u003cbr\u003e\u003cul data-sourcepos=\"17:5-19:65\"\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"17:5-17:33\"\u003eSoluzione di etanolo al 60%\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"18:5-19:65\"\u003eCocktail ad alta concentrazione di mercaptoetanolo (metodo di Mendez) L'estrazione avviene tramite agitazione su shaker o rotatore.\u003c\/li\u003e\n\u003cbr\u003e\n\u003c\/ul\u003e\n\u003cbr\u003e\n\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"20:1-20:81\"\u003e\n\u003cstrong\u003eDiluizione:\u003c\/strong\u003e L'estratto viene diluito in soluzione salina tamponata (PBS).\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"21:1-21:186\"\u003e\n\u003cstrong\u003eLegame (Cattura):\u003c\/strong\u003e I campioni diluiti sono aggiunti a pozzetti rivestiti con anticorpi R5 (anticorpo di cattura). La gliadina presente si lega all'anticorpo durante l'incubazione.\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"22:1-22:124\"\u003e\n\u003cstrong\u003eRilevazione:\u003c\/strong\u003e Un secondo anticorpo R5 (anticorpo rivelatore), marcato con un enzima, si lega alla gliadina catturata.\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"23:1-26:0\"\u003e\n\u003cstrong\u003eSviluppo del Colore:\u003c\/strong\u003e In presenza di un substrato specifico, l'enzima sviluppa una colorazione proporzionale alla concentrazione di glutine.\u003cbr\u003e\u003cul data-sourcepos=\"24:5-26:0\"\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"24:5-24:47\"\u003e\n\u003cstrong\u003eBlu:\u003c\/strong\u003e Indica alti livelli di gliadina.\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"25:5-26:0\"\u003e\n\u003cstrong\u003eViola\/Rosso:\u003c\/strong\u003e Indica bassi o nulli livelli di gliadina.\u003c\/li\u003e\n\u003cbr\u003e\n\u003c\/ul\u003e\n\u003cbr\u003e\n\u003c\/li\u003e\n\u003cbr\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp data-sourcepos=\"27:1-27:23\"\u003e\u003cstrong\u003eFornito con il Kit:\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cul data-sourcepos=\"29:1-31:0\"\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"29:1-29:64\"\u003eSet di standard per la creazione di una curva di calibrazione.\u003c\/li\u003e\n\u003cbr\u003e\u003cli data-sourcepos=\"30:1-31:0\"\u003eEsclusivo foglio di calcolo per l'analisi dei dati.\u003c\/li\u003e\n\u003cbr\u003e\n\u003c\/ul\u003e\u003cbr\u003e\u003cp data-sourcepos=\"32:1-32:206\"\u003e\u003cstrong\u003eAnalisi Avanzata:\u003c\/strong\u003e Inserendo le densità ottiche dei campioni e degli standard, il foglio di calcolo determina l'esatta concentrazione di gliadina in ppm tramite interpolazioni multiparametriche avanzate.\u003c\/p\u003e\u003cbr\u003e","brand":"vendor-unknown","offers":[{"title":"Default Title","offer_id":56592889512262,"sku":null,"price":320.16,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0998\/2502\/5350\/files\/kit_neogen-scaled.jpg?v=1778853451","url":"https:\/\/deltacon-store.myshopify.com\/products\/veratox-r5-gliadin-elisa-ricerca-glutine","provider":"Deltacon S.r.l","version":"1.0","type":"link"}